Maryam Parviz; Mohammad Rabiee; Mohammad Ali Shokrgozar; Fathollah Moztarzadeh; Farzad Yousefi Gharebaghi; Mahdis Shayan; Mohammad Reza Tahriri
Volume 3, Issue 2 , June 2009, , Pages 89-97
Abstract
A great deal of attention has been drawn to the colloidal chemistry based semiconductor nanocrysallites, also known as quantum dots (QDs). Because of the strong quantum confinement, quantum dots have unique size-dependent optical properties, which are much more superior to the conventional organic fluorescence ...
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A great deal of attention has been drawn to the colloidal chemistry based semiconductor nanocrysallites, also known as quantum dots (QDs). Because of the strong quantum confinement, quantum dots have unique size-dependent optical properties, which are much more superior to the conventional organic fluorescence materials. In addition, strong chemical resistant makes inorganic semiconductor QDs an ideal candidate for next-generation of bio-labels and other biomedical applications. This study presents a synthesize method of high quality biocompatible CdSe QDs in aqueous solution by using gelatin as inhibitor. A thin ZnS layer was grown on CdSe QDs, forming a CdSe/ZnS core/shell structure, to improve the photoluminescence. The optical properties of the QDs were characterized by photoluminescence spectra. The stability of CdSe/ZnS QDs in aqueous solution has been improved with capping in mercaptosuccinic acid (MSA) groups. PAGE test results show that carboxylic groups of MSA have interacted with NH2 agent of CD20 antibodies. We evaluate the in vitro efficiency of the luminescence of the bioconjugated QDs to kill Raji cells. Raji cells have anti-CD20 which can especially connect to CD20-QDs. In conclusion, we found that CD20-CdSe/ZnS bioconjugated QDs with different concentration 50-250 µg/mL which have been exited at 400nm in 2h intervals can result in Raji cells death. This study shows the potential of CdSe/ZnS for cancer treatment.
